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phluc reporter plasmids  (Addgene inc)


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    Structured Review

    Addgene inc phluc reporter plasmids
    Overview of the <t>pHLuc</t> system and experimental workflow. The <t>pHLuc</t> <t>reporter</t> is a multicistronic cassette that comprises of cytoplasmic Antares, T2A-puromycin, a mutated internal ribosome entry site (IRES) v24, and a membrane-bound SEPLuc that utilizes a weaker variant of Nanoluc. Cells transfected with pHLuc have shown that the reporter is pH-responsive compared to a pH-stable control. Since the SEP signal is pH-dependent, the ratio of SEP to Antares (R 580 / 510 ) is indicative of changes in extracellular pH. When cells stably expressing pHLuc were injected into mice, bioluminescence imaging also showed differences in the ratiometric readout. In vivo ratiometric images showed the capacity of pHLuc to detect extracellular pH changes. Image generated via BioRender.
    Phluc Reporter Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phluc reporter plasmids/product/Addgene inc
    Average 91 stars, based on 1 article reviews
    phluc reporter plasmids - by Bioz Stars, 2026-05
    91/100 stars

    Images

    1) Product Images from "pHLuc, a Ratiometric Luminescent Reporter for in vivo Monitoring of Tumor Acidosis"

    Article Title: pHLuc, a Ratiometric Luminescent Reporter for in vivo Monitoring of Tumor Acidosis

    Journal: Frontiers in Bioengineering and Biotechnology

    doi: 10.3389/fbioe.2020.00412

    Overview of the pHLuc system and experimental workflow. The pHLuc reporter is a multicistronic cassette that comprises of cytoplasmic Antares, T2A-puromycin, a mutated internal ribosome entry site (IRES) v24, and a membrane-bound SEPLuc that utilizes a weaker variant of Nanoluc. Cells transfected with pHLuc have shown that the reporter is pH-responsive compared to a pH-stable control. Since the SEP signal is pH-dependent, the ratio of SEP to Antares (R 580 / 510 ) is indicative of changes in extracellular pH. When cells stably expressing pHLuc were injected into mice, bioluminescence imaging also showed differences in the ratiometric readout. In vivo ratiometric images showed the capacity of pHLuc to detect extracellular pH changes. Image generated via BioRender.
    Figure Legend Snippet: Overview of the pHLuc system and experimental workflow. The pHLuc reporter is a multicistronic cassette that comprises of cytoplasmic Antares, T2A-puromycin, a mutated internal ribosome entry site (IRES) v24, and a membrane-bound SEPLuc that utilizes a weaker variant of Nanoluc. Cells transfected with pHLuc have shown that the reporter is pH-responsive compared to a pH-stable control. Since the SEP signal is pH-dependent, the ratio of SEP to Antares (R 580 / 510 ) is indicative of changes in extracellular pH. When cells stably expressing pHLuc were injected into mice, bioluminescence imaging also showed differences in the ratiometric readout. In vivo ratiometric images showed the capacity of pHLuc to detect extracellular pH changes. Image generated via BioRender.

    Techniques Used: Membrane, Variant Assay, Transfection, Control, Stable Transfection, Expressing, Injection, Imaging, In Vivo, Generated



    Similar Products

    phluc reporter plasmids  (Addgene inc)
    91
    Addgene inc phluc reporter plasmids
    Overview of the <t>pHLuc</t> system and experimental workflow. The <t>pHLuc</t> <t>reporter</t> is a multicistronic cassette that comprises of cytoplasmic Antares, T2A-puromycin, a mutated internal ribosome entry site (IRES) v24, and a membrane-bound SEPLuc that utilizes a weaker variant of Nanoluc. Cells transfected with pHLuc have shown that the reporter is pH-responsive compared to a pH-stable control. Since the SEP signal is pH-dependent, the ratio of SEP to Antares (R 580 / 510 ) is indicative of changes in extracellular pH. When cells stably expressing pHLuc were injected into mice, bioluminescence imaging also showed differences in the ratiometric readout. In vivo ratiometric images showed the capacity of pHLuc to detect extracellular pH changes. Image generated via BioRender.
    Phluc Reporter Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phluc reporter plasmids/product/Addgene inc
    Average 91 stars, based on 1 article reviews
    phluc reporter plasmids - by Bioz Stars, 2026-05
    91/100 stars
    		  Buy from Supplier

    Image Search Results


    Overview of the pHLuc system and experimental workflow. The pHLuc reporter is a multicistronic cassette that comprises of cytoplasmic Antares, T2A-puromycin, a mutated internal ribosome entry site (IRES) v24, and a membrane-bound SEPLuc that utilizes a weaker variant of Nanoluc. Cells transfected with pHLuc have shown that the reporter is pH-responsive compared to a pH-stable control. Since the SEP signal is pH-dependent, the ratio of SEP to Antares (R 580 / 510 ) is indicative of changes in extracellular pH. When cells stably expressing pHLuc were injected into mice, bioluminescence imaging also showed differences in the ratiometric readout. In vivo ratiometric images showed the capacity of pHLuc to detect extracellular pH changes. Image generated via BioRender.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: pHLuc, a Ratiometric Luminescent Reporter for in vivo Monitoring of Tumor Acidosis

    doi: 10.3389/fbioe.2020.00412

    Figure Lengend Snippet: Overview of the pHLuc system and experimental workflow. The pHLuc reporter is a multicistronic cassette that comprises of cytoplasmic Antares, T2A-puromycin, a mutated internal ribosome entry site (IRES) v24, and a membrane-bound SEPLuc that utilizes a weaker variant of Nanoluc. Cells transfected with pHLuc have shown that the reporter is pH-responsive compared to a pH-stable control. Since the SEP signal is pH-dependent, the ratio of SEP to Antares (R 580 / 510 ) is indicative of changes in extracellular pH. When cells stably expressing pHLuc were injected into mice, bioluminescence imaging also showed differences in the ratiometric readout. In vivo ratiometric images showed the capacity of pHLuc to detect extracellular pH changes. Image generated via BioRender.

    Article Snippet: The pHLuc reporter plasmids constructed in this work are available from Addgene.

    Techniques: Membrane, Variant Assay, Transfection, Control, Stable Transfection, Expressing, Injection, Imaging, In Vivo, Generated